Human EPO R ELISA Kit

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Human EPO R ELISA Kit
Human EPO R ELISA Kit Standard Curve

Human EPO R ELISA Kit

Catalog No : 3010188


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SKU: 3010188. Categories: , , , .

Human EPO R ELISA Kit Features

Product Name            Human EPO R ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   100 pg/mL
Assay Range                187.5-12000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               EPOR, EPO-R
Gene ID                         2057
Swissprot ID              P19235

Recovery

Sample Type    Average % RecoveryRange %
Serum  8779-93
Plasma  9580-113
Cell culture media  9679-124

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:495
1:897
Plasma1:297
1:493
1:8101
Cell culture media1:292
1:486
1:894

Standard Curve: Human EPO R ELISA Kit

Human EPO R ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cytoplasmic domains of the EpoR contain a number of phosphotyrosines that are phosphorylated by Jak2 and serve as docking sites for a variety of intracellular pathway activators and Stats. In addition to activating Ras/AKT and ERK/MAP kinase, phosphatidylinositol 3-kinase/AKT pathway and STAT transcription factors, phosphotyrosines also serve as docking sites for phosphatases that negatively affect EpoR signaling in order to prevent overactivation that may lead to such disorders as erythrocytosis. In general, the defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Mutations in Jak2 kinases associated with EpoR can also lead to polycythemia vera.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human EPO R ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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