Human Endoglin ELISA Kit

Human Endoglin ELISA Kit Features

Product Name           Human Endoglin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   10 pg/ml
Assay Range               12.4-3000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ENG, END, HHT1, ORW1
Gene ID                         2022
Swissprot ID              P17813

Recovery

Linearity

Standard Curve: Human Endoglin ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida family of proteins. Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane and cytoplasmic domains. It is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3, but only after binding T beta RII.

Similarly, they interact with Activin A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-IA/ALK-3 or BMPR-IB/ALK-6. BMP-9, however, is reported to bind endoglin directly. Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP 7 signals in the same myoblast cell line. In endothelial cells, endoglin inhibits T beta RI/ALK 5, but enhances ALK 1-mediated activation. In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (Flt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity.

Related Products

Mouse Endoglin ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Endoglin ELISA Kit is for Research Use Only.

• Manual
• Assay procedure
• MSDS