Human EG-VEGF ELISA Kit

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Human EG-VEGF ELISA Kit
Human EG-VEGF ELISA Kit Standard Curve

Human EG-VEGF ELISA Kit

Catalog No : 3010183


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SKU: 3010183. Categories: , .

Human EG-VEGF ELISA Kit Features

Product Name            Human EG-VEGF ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   8 pg/mL
Assay Range                13.7-10000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               PROK1, EGVEGF, PK1, PRK1
Gene ID                         84432
Swissprot ID               P58294

Recovery

Sample Type    Average % RecoveryRange %
Serum   9080-101
Plasma   7970-89
Cell culture media  7872-94

Linearity

Sample TypeDilution% of Expected
Serum1:2117
1:495
1:8108
Plasma1:2121
1:484
1:895
Cell culture media1:2119
1:497
1:894

Standard Curve: Human EG-VEGF ELISA Kit

Human EG-VEGF ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Endocrine gland-derived vascular endothelial growth factor (EG-VEGF), also called prokineticin 1 (PK1), is a member of the prokineticin family of secreted proteins that share a common structural motif containing ten conserved cysteine residues that form five pairs of disulfide bonds. EG-VEGF is expressed in multiple tissues including the gastrointestinal tract and steroidogenic glands. EG-VEGF has been shown to potently stimulate the contraction of GI smooth muscle.

In addition, EG-VEGF is a tissue-specific angiogenic factor that exhibits biological activities similar to that of VEGF on select cells. It induces the proliferation, migration, and fenestration in cultured endocrine gland-derived capillary endothelial cells. EG-VEGF binds to and activates two closely related G protein-coupled receptors, EG-VEGF/PK1-R1 and EG-VEGF/PK2-R2. Activation of the receptors leads to stimulation of phosphoinositide turnover and activation of p44/p42 MAP kinase signaling pathways.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human EG-VEGF ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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