Human E-Selectin ELISA Kit

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Human E-Selectin ELISA Kit
Human E-Selectin ELISA Kit Standard Curve

Human E-Selectin ELISA Kit

Catalog No : 3010061


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Human E-Selectin ELISA Kit Features

Product Name           Human E-Selectin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   15 pg/ml
Assay Range               24.7-18000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               SELE; CD62E; ELAM; ELAM1; ESEL; LECAM2
Gene ID                         6401
Swissprot ID               P16581

Recovery

Sample Type    Average % RecoveryRange %
Serum 9585-106
Plasma 9687-110
Cell culture media 9382-107

Linearity

Sample TypeDilution% of Expected
Serum1:293
1:4101
1:896
Plasma1:292
1:499
1:895
Cell culture media1:296
1:4103
1:8101

Standard Curve: Human E-Selectin ELISA Kit

Human E-Selectin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. L-Selectin is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human E-Selectin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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