Human DLL1 ELISA Kit

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Human DLL1 ELISA Kit
Human DLL1 ELISA Kit Standard Curve

Human DLL1 ELISA Kit

Catalog No : 3010178


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SKU: 3010178. Categories: , , .

Human DLL1 ELISA Kit Features

Product Name            Human DLL1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetrichttps://bioaimscientific.com/wp-admin/options-general.php?page=wprocket
Sensitivity                   25 pg/mL
Assay Range                49.2-12000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               DLL1, DELTA1, DL1, Delta
Gene ID                         28514
Swissprot ID               O00548

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   10194-115
Plasma   9283-105
Cell culture media   9085-102

Linearity

Sample TypeDilution% of Expected
Serum1:2110
1:490
1:892
Plasma1:2117
1:489
1:897
Cell culture media1:2109
1:485
1:896

Standard Curve: Human DLL1 ELISA Kit

Human DLL1 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in central nervous system morphogenesis, intervertebral joint formation, inner ear hair cell development and patterning, and arteriogenesis. It regulates the homeostasis and differentiation of neural, pancreatic, and myogenic progenitor cells, T and B lymphocytes. DLL1 is overexpressed in a number of cancers and contributes to tumorigenesis. The extracellular region of DLL1 can be shed from the cell surface, and the intracellular domain can be cleaved by gamma-secretase to translocate to the nucleus and regulate gene transcription. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human DLL1 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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