Human Dkk-1 ELISA Kit

Human Decorin ELISA Kit
Human Decorin ELISA Kit
April 9, 2016
Human Dkk-3 ELISA Kit
Human Dkk-3 ELISA Kit
April 9, 2016
Show all
Human Dkk-1 ELISA Kit
Human Dkk-1 ELISA Kit Standard Curve

Human Dkk-1 ELISA Kit

Catalog No : 3010054


Clear

Human Dkk-1 ELISA Kit Features

Product Name           Human Dkk-1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   80 pg/ml
Assay Range               122.9-30000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               DKK1; DKK-1; SK
Gene ID                        22943
Swissprot ID              O94907

Recovery

Sample Type    Average % RecoveryRange %
Serum 9080-101
Plasma 8178-89
Cell culture media 10191-112

Linearity

Sample TypeDilution% of Expected
Serum1:296
1:492
1:889
Plasma1:297
1:494
1:8101
cell culture media1:2102
1:490
1:8103

Standard Curve: Human Dkk-1 ELISA Kit

Human Dkk-1 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Dickkopf WNT signaling pathway inhibitor 1 (Dkk1) is a protein-coding gene that acts from the anterior visceral endoderm. The dickkopf protein encoded by DKK1 is an antagonistic inhibitor of the WNT signaling pathway that acts by isolating the LRP6 co-receptor so that it cannot aid in activating the WNT signaling pathway. DKK1 was also demonstrated to antagonize the Wnt/β-catenin pathway via a reduction in β-catenin and an increase in OCT4 expression. This inhibition plays a key role in heart, head and forelimb development during anterior morphogenesis of the embryo.

Related Products

Human Dkk-3 ELISA Kit

Human Dkk-4 ELISA Kit

Mouse Dkk-1 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Dkk-1 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Reviews

There are no reviews yet.

Be the first to review “Human Dkk-1 ELISA Kit”