Human Decorin ELISA Kit

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Human Decorin ELISA Kit
Human Decorin ELISA Kit Standard Curve

Human Decorin ELISA Kit

Catalog No : 3010053


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Human Decorin ELISA Kit Features

Product Name           Human Decorin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   < 1 pg/ml
Assay Range               0.96-700 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CSCD; DSPG2; PG40; PGII; PGS2; SLRR1B
Gene ID                        1634
Swissprot ID              P07585

Recovery

Sample Type    Average % RecoveryRange %
Serum 119108-128
Plasma 111102-122
Cell culture media 9484-104

Linearity

Sample TypeDilution% of Expected
Serum1:299
1:481
1:8101
Plasma1:2101
1:489
1:896
cell culture media1:2107
1:492
1:898

Standard Curve: Human Decorin ELISA Kit

Human Decorin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Decorin is a proteoglycan that is on average 90 – 140 kDa. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Decorin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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