Catalog No : 3010053
Product Name Human Decorin ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100 ul
Detection Method Colorimetric
Sensitivity < 1 pg/ml
Assay Range 0.96-700 pg/ml
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names CSCD; DSPG2; PG40; PGII; PGS2; SLRR1B
Gene ID 1634
Swissprot ID P07585
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 119 | 108-128 |
Plasma | 111 | 102-122 |
Cell culture media | 94 | 84-104 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 99 |
1:4 | 81 | |
1:8 | 101 | |
Plasma | 1:2 | 101 |
1:4 | 89 | |
1:8 | 96 | |
cell culture media | 1:2 | 107 |
1:4 | 92 | |
1:8 | 98 |
Standard Curve: Human Decorin ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Decorin is a proteoglycan that is on average 90 – 140 kDa. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin promotes myoblast differentiation, supports angiogenesis, and inhibits tumor progression.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human Decorin ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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