Catalog No : 3010050
Product Name Human CT-1 ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100 ul
Detection Method Colorimetric
Sensitivity 10 pg/ml
Assay Range 16.5-12000 pg/ml
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names: CTF1; CT-1; CT1
Gene ID 1489
Swissprot ID Q16619
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 120 | 115-132 |
Plasma | 101 | 85-124 |
Cell culture media | 99 | 82-107 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 96 |
1:4 | 80 | |
1:8 | 87 | |
Plasma | 1:2 | 121 |
1:4 | 102 | |
1:8 | 91 | |
Cell culture media | 1:2 | 102 |
1:4 | 115 | |
1:8 | 90 |
Standard Curve: Human CT-1 ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human CT-1 ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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