Human Clusterin ELISA Kit

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Human Clusterin ELISA Kit
Human Clusterin ELISA Kit Standard Curve

Human Clusterin ELISA Kit

Catalog No : 3010049


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Human Clusterin ELISA Kit Features

Product Name           Human Clusterin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   4 pg/ml
Assay Range               4.9-20000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               AAG4; APO-J; APOJ; CLI1;CLU2; KUB1; NA1/NA2; SGP2; SP-40; TRPM-2; TRPM2; CLU
Gene ID                         1191
Swissprot ID               P10909

Recovery

Sample Type    Average % RecoveryRange %
Serum 119109-135
Plasma 115104-128
Cell culture media 109102-115

Linearity

Sample TypeDilution% of Expected
Serum1:2110
1:4106
1:8102
Plasma1:2108
1:4120
1:8113
Cell culture media1:2108
1:4117
1:8104

Standard Curve: Human Clusterin ELISA Kit

Human Clusterin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Clusterin is a secreted multifunctional protein that binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. CLU is a molecular chaperone responsible for aiding protein folding of secreted proteins. Its three isoforms have been differentially implicated in pro- or antiapoptotic processes. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis. Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Clusterin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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