Human CD229 ELISA Kit

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Human CD229 ELISA Kit
Human CD229 ELISA Kit Standard Curve

Human CD229 ELISA Kit

Catalog No : 3010168


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Human CD229 ELISA Kit Features

Product Name            Human CD229 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   50 pg/mL
Assay Range                81.9-20000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               LY9, CD229, SLAMF3, hly9, mLY9
Gene ID                         4063
Swissprot ID              Q9HBG7

Recovery

Sample Type    Average % RecoveryRange %
Serum   11595-135
Plasma   120112-136
Cell culture media   119109-128

Linearity

Sample TypeDilution% of Expected
Serum1:2122
1:4119
1:8118
Plasma1:2125
1:4120
1:8114
Cell culture media1:2127
1:4121
1:8110

Standard Curve: Human CD229 ELISA Kit

Human CD229 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. CD229 is expressed on T and B cells, thymocytes, and more weakly on NK cells. Homophilic binding between CD229 molecules is mediated by the N-terminal Ig‑like domain. Human and mouse CD229 exhibit cross-species binding. Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell – B cell contact. Two tyrosines in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP-2 (μ2 chain) and GRB-2 and both are required for CD229 internalization. In addition, there are two ITSMs which mediate phosphorylation-dependent CD229 association with SAP and SHP-2.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human CD229 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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