Human CD200 ELISA Kit

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Human CD200 ELISA Kit
Human CD200 ELISA Kit Standard Curve

Human CD200 ELISA Kit

Catalog No : 3010167


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SKU: 3010167. Categories: , .

Human CD200 ELISA Kit Features

Product Name            Human CD200 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   18 pg/mL
Assay Range                24.6-6000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               CD200, MOX1, MOX2, MRC, OX-2
Gene ID                         4345
Swissprot ID               P41217

Recovery

Sample Type    Average % RecoveryRange %
Serum   8678-97
Plasma   8277-93
Cell culture media   8478-94

Linearity

Sample TypeDilution% of Expected
Serum1:2120
1:4119
1:8108
Plasma1:2121
1:4117
1:8118
Cell culture media1:2123
1:4121
1:8109

Standard Curve: Human CD200 ELISA Kit

Human CD200 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CD200 antigen, also known as OX-2, is a type I transmembrane protein belonging to the Ig superfamily. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. CD200 is widely expressed in multiple cell types. It interacts with CD200 R1, another Ig superfamily inhibitory receptor primarily expressed on leukocytes of the myeloid lineage, to inhibit myeloid functions. CD200 R1 is a transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed primarily in cells of the myeloid lineage, while its ligand, CD200 R1 is though to propagate inhibitory signals and disruption of the CD200/CD200 R1 receptor-ligand pair leads to a predisposition to autoimmune phenotypes.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human CD200 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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