Catalog No : 3010157
Product Name Human BCAM ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity 20 pg/mL
Assay Range 40.9-10000 pg/mL
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names BCAM, AU, CD239, LU, MSK19
Gene ID 4059
Swissprot ID P50895
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 78 | 72-89 |
Plasma | 86 | 80-94 |
Cell culture media | 88 | 81-97 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 110 |
1:4 | 120 | |
1:8 | 118 | |
Plasma | 1:2 | 112 |
1:4 | 102 | |
1:8 | 115 | |
Cell culture media | 1:2 | 114 |
1:4 | 117 | |
1:8 | 122 |
Standard Curve: Human BCAM ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Basal cell adhesion molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily protein that differ in the length of their cytoplasmic tails. Lutheran blood group glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Two transcript variants encoding different isoforms have been found for this gene.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human BCAM ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
---|---|
Species Detected | Human |
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