Human ApoA2 ELISA Kit

Human ApoA1 ELISA Kit
July 15, 2017
Human ApoB100 ELISA Kit
July 16, 2017
Show all
Human ApoA2 ELISA Kit

Human ApoA2 ELISA Kit

Catalog No : 3010384

SKU: 3010384. Categories: , , .

Human ApoA2 ELISA Kit Features

Product Name            Human ApoA2 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                    25 pg/mL
Assay Range                0.027-20 ng/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               APOA2, Apo-AII, ApoA-II, apoAII
Gene ID                         336
Swissprot ID               P02652


Sample Type    Average % RecoveryRange %
Serum   11790-130
Plasma   12298-134
cell culture media   11092-128


Sample TypeDilution% of Expected
cell culture media1:2108

 Standard Curve: Human ApoA2 ELISA Kit

Human ApoA2 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.


Apolipoprotein A-II is a protein that is encoded by the APOA2 gene. It is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. APOA2 has been shown to interact with PLTP. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human ApoA2 ELISA Kit is for Research Use Only.


1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected



There are no reviews yet.

Be the first to review “Human ApoA2 ELISA Kit”