Human Angiopoietin-1 ELISA Kit

Human Angiopoietin-1 ELISA Kit Features

Product Name          Human Angiopoietin-1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   15pg/mL
Assay Range                24.7-18000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ANGPT1; AGP1; AGPT; ANG1
Gene ID                         284
Swissprot ID               Q15389

Recovery

Linearity

 Standard Curve: Human Angiopoietin-1 ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Angiopoietins are proteins with important roles in vascular development and angiogenesis, which bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Ang-1, Ang-2, and Ang-3 and Ang-4 are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase. They are important modulators of angiogenesis. It plays a critical role in mediating reciprocal interactions between the endothelium and mesenchyme. Ang-1 contributes to blood vessel maturation and stability; it also may be involved in early development of the heart.

Related Products

Human Angiopoietin-1 EasyTest™ ELISA Kit

Human Angiopoietin-2 ELISA Kit

Human Angiopoietin-4 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Angiopoietin-1 ELISA Kit is for Research Use Only.

• Manual
• Assay procedure
• MSDS