Catalog No : 3010002
Product Name Human Angiopoietin-1 ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity 15pg/mL
Assay Range 24.7-18000 pg/mL
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names ANGPT1; AGP1; AGPT; ANG1
Gene ID 284
Swissprot ID Q15389
Recovery
Sample Type | Average % Recovery | Range % |
Serum | 95 | 83-103 |
Plasma | 94 | 80-102 |
cell culture media | 96 | 85-107 |
Linearity
Sample Type | Dilution | % of Expected |
Serum | 1:2 | 94 |
1:4 | 97 | |
1:8 | 94 | |
Plasma | 1:2 | 93 |
1:4 | 95 | |
1:8 | 98 | |
cell culture media | 1:2 | 95 |
1:4 | 92 | |
1:8 | 91 |
Standard Curve: Human Angiopoietin-1 ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Angiopoietins are proteins with important roles in vascular development and angiogenesis, which bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Ang-1, Ang-2, and Ang-3 and Ang-4 are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase. They are important modulators of angiogenesis. It plays a critical role in mediating reciprocal interactions between the endothelium and mesenchyme. Ang-1 contributes to blood vessel maturation and stability; it also may be involved in early development of the heart.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Human Angiopoietin-1 ELISA Kit is for Research Use Only.
Sizes | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
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