Human Angiopoietin-1 EasyTest™ ELISA Kit

Product Features

Product Name              Human Angiopoietin-1 EasyTest™ ELISA Kit
Assay Type                      Solid Phase Sandwich ELISA
Format                             96-well strip plate
Assay Process               One wash step, two incubations
Assay Length                1.5 hours
Sample Volume           Serum , Plasma, 50µL
Detection Method       Colorimetric
Sensitivity                     17pg/mL
Assay Range                  98.3-24000pg/mL
Reactivity                        Human
Cross-Reactivity          No cross-reactivity observed with available related molecules
Gene Names                  ANGPT1; AGP1; AGPT; ANG1
Gene ID                            284
Swissprot ID                 Q15389

Recovery

Linearity

Standard Curve: Human Angiopoietin-1 EasyTest™ ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human Angiopoietin-1 mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2 The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Angiopoietin-1 EasyTest™ ELISA Kit is for Research Use Only.

• Manual
• AssayProcedure
• MSDS