Human Angiogenin ELISA Kit

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Human Angiogenin ELISA Kit
Human Angiogenin ELISA Kit Standard Curve

Human Angiogenin ELISA Kit

Catalog No : 3010032


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SKU: 3010032. Categories: , .

Human Angiogenin ELISA Kit Features

Product Name            Human Angiogenin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   < 1 pg/ml
Assay Range               1.6-400 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ANG, ALS9, HEL168, RAA1, RNASE4, RNASE5
Gene ID                        283
Swissprot ID              P03950

Recovery

Sample Type    Average % RecoveryRange %
Serum 9682-106
Plasma  9484-104
Cell culture media 9585-104

Linearity

Sample TypeDilution% of Expected
Serum1:297
1:496
1:894
Plasma1:296
1:495
1:8101
Cell culture media1:296
1:495
1:897

Standard Curve: Human Angiogenin ELISA Kit

Human Angiogenin ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Angiogenin (Ang) also known as ribonuclease 5 is a small 123 amino acid protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessels through the process of angiogenesis. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human Angiogenin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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