Human ALCAM ELISA Kit

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Human ALCAM ELISA Kit
Human ALCAM ELISA Kit Standard Curve

Human ALCAM ELISA Kit

Catalog No : 3010153


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Human ALCAM ELISA Kit Features

Product Name            Human ALCAM ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   10 pg/mL
Assay Range                20.5-5000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               ALCAM, CD166, MEMD
Gene ID                         214
Swissprot ID              Q13740

Recovery

Sample Type    Average % RecoveryRange %
Serum   9990-107
Plasma   9288-106
Cell culture media   110101-122

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:497
1:893
Plasma1:2101
1:480
1:885
Cell culture media1:2103
1:492
1:889

Standard Curve: Human ALCAM ELISA Kit

Human ALCAM ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Activated leukocyte cell adhesion molecule (ALCAM) is a typeI membrane glycoprotein that is a member of the immunoglobulin superfamily. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. In humans, it is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human ALCAM ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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