Human Activin A ELISA Kit

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Human Activin A ELISA Kit
Human Activin A ELISA Kit Standard Curve

Human Activin A ELISA Kit

Catalog No : 3010029


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SKU: 3010029. Categories: , , .

Human Activin A ELISA Kit Features

Product Name           Human Activin A ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100 ul
Detection Method    Colorimetric
Sensitivity                   10 pg/ml
Assay Range               20.5-5000 pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               INHBA
Gene ID                        3624
Swissprot ID              P08476

Recovery

Sample Type    Average % RecoveryRange %
Serum 8078-89
Plasma  7872-93
Cell culture media  8277-94

Linearity

Sample TypeDilution% of Expected
Serum1:287
1:4117
1:8101
Plasma1:2125
1:4110
1:8105
Cell culture media1:298
1:4107
1:8101

Standard Curve: Human Activin A ELISA Kit

Human Activin A ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair, and endocrine function. Conversely, inhibin downregulates FSH synthesis and inhibits FSH secretion.

Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A – beta A), Activin B (beta B – beta B), Activin AB (beta A – beta B), Inhibin A (alpha – beta A) and Inhibin B (alpha – beta B).

Related Products

Custom mouse Activin A ELISA Kit

Rat Activin A ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human Activin A ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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