Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
WB IHC IF
Hu Ms Rt
The antibody detects endogenous level of GSK3a only when phosphorylated at serine 21and does not detect GSK-3b when phosphorylated at Ser9.
Peptide-KLH. Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a.
Factor A; GSK-3 alpha; kinase GSK3-alpha
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.
Western blotting: 1:500~1:1000
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.
Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
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Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.