Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Hu Ms Rt
The antibody detects endogenous level of Elk-1 only when phosphorylated at serine 383.
Peptide-KLH. Peptide sequence around phosphorylation site of serine 383 (T-L-S(p)-P-I) derived from Human Elk-1.
ELK1; ETS-domain protein Elk-1
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.
Western blotting: 1:500~1:1000
Transcription factor that recognizes and binds to the enhancer heptamer motif 5′-TGA[CG]TCA-3′.