Custom rat IL-1 R6 ELISA Kit

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rat IL-1 R6 ELISA Kit

Custom rat IL-1 R6 ELISA Kit

Catalog No : 3030225


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SKU: 3030225. Category: .

Product Features

Product Name            Custom rat IL-1 R6 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   This has not yet been determined
Assay Range                This has not yet been determined
Reactivity                     Rat
Cross-Reactivity       This ELISA antibody pair only recognizes rat IL-1 R6
Gene Names:              Il1rl2
Gene ID                         171106
Swissprot ID               Q62929

 

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL 1 R3), ST2 (T1/IL-1 R4), IL 18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL 1 (TIGIRR 2/IL 1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells.

IL 1 Rrp2 has been shown to mediate the activation of the transcription factor NF kB by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1e), with IL 1 RAcP as a co-factor. Response to IL-1 F9 is specifically antagonized by IL-1 F5, an IL-1 family ligand that is most closely related to IL 1ra. IL-1 Rrp2, IL-1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses.

Related Products

Human IL-1 R6 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Custom rat IL-1 R6 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Rat

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  • Manual
  • Assay procedure
  • MSDS