Custom mouse Prostasin ELISA Kit

Product Features

Product Name           Custom mouse Prostasin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   This has not yet been determined
Assay Range                This has not yet been determined
Reactivity                     Mouse
Cross-Reactivity       This ELISA antibody pair only recognizes mouse Prostasin
Gene Names:              Prss8; 2410039E18Rik; AI313909; C79772; CAP1; fr; mCAP1
Gene ID                         76560
Swissprot ID               Q9ESD1

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Prostasin, also known as channel activating protease 1, is a serine protease with trypsin-like substrate specificity. The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. It is highly expressed in the prostate gland, and is also found at lower levels in the lung, kidney, liver, salivary gland, and pancreas. The peptidase activity of prostasin is involved in the regulation of epithelial sodium channels.

Related Products

Human Prostasin ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Custom mouse Prostasin ELISA Kit is for Research Use Only.

• Manual
• Assay procedure
• MSDS