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mouse IL-7 R alpha ELISA Kit

Custom mouse IL-7 R alpha ELISA Kit

Catalog No : 3020507


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SKU: 3020507. Category: .

Product Features

Product Name            Custom mouse IL-7 R alpha ELISA Kit 
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   This has not yet been determined
Assay Range                This has not yet been determined
Reactivity                     Mouse
Cross-Reactivity       This ELISA antibody pair only recognizes mouse IL-7 R alpha
Gene Names:              Il7r; CD127; IL-7Ralpha
Gene ID                         16197
Swissprot ID               P16872

 

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7 R alpha associates with the common gamma chain to form the functional high affinity IL-7 receptor complex. IL 7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors. It is expressed early in B cell development, prior to the appearance of surface IgM.

In mouse, IL-7 activation of IL 7 R alpha is critical for both T cell and B cell lineage development. In human it is required for T cell but not for B cell development. IL 7 induces the down regulation and shedding of cell surface IL-7 R alpha. IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin. TSLP indirectly regulates T cell development by modulating dendritic cell activation. The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL 7 R alpha to form functional complexes with SCF R and HGF R.

Related Products

Mouse IL-7 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Custom mouse IL-7 R alpha ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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  • Manual
  • Assay procedure
  • MSDS