Custom mouse IL-3 R beta ELISA Kit

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mouse IL-3 R beta ELISA Kit

Custom mouse IL-3 R beta ELISA Kit

Catalog No : 3020504


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SKU: 3020504. Category: .

Product Features

Product Name            Custom mouse IL-3 R beta ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   This has not yet been determined
Assay Range                This has not yet been determined
Reactivity                     Mouse
Cross-Reactivity       This ELISA antibody pair only recognizes mouse IL-3 R beta
Gene Names:              Csf2rb2; AIC2A; BetaIl3; Bil3; Csfgmrb; Il3r; Il3rb; Il3rb2
Gene ID                         12984
Swissprot ID               P26954

 

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity. The second mIL-3 R beta was referred to as AIC2B .

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Mouse IL-3 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Custom mouse IL-3 R beta ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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  • Manual
  • Assay procedure
  • MSDS