Canine VEGF-A ELISA Kit

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Canine VEGF-A ELISA Kit

Canine VEGF-A ELISA Kit

Catalog No : 3060015


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SKU: 3060015. Category: .

Canine VEGF-A ELISA Kit Features

Product Name           Canine VEGF-A ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   5 pg/mL
Assay Range                6.1-1500 pg/mL
Reactivity                     Canine
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               VEGFA; VEGF
Gene ID                         403802
Swissprot ID               Q9MYV3

Recovery

Sample Type    Average % RecoveryRange %
Serum 8370-105
Plasma 7269-81
Cell culture media 11495-132

Linearity

Sample TypeDilution% of Expected
Serum1:293
1:483
1:887
Plasma1:287
1:486
1:885
Cell culture media1:2103
1:4105
1:8110

Standard Curve: Canine VEGF-A ELISA Kit

Canine VEGF-A ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

VEGF is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. All members of the VEGF family stimulate cellular responses by binding to tyrosine kinase receptors (the VEGFRs) on the cell surface, causing them to dimerize and become activated through transphosphorylation. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF. The function of VEGFR-1 is less well-defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding. VEGF-C and VEGF-D, but not VEGF-A, are ligands for a third receptor (VEGFR-3/Flt4), which mediates lymphangiogenesis.

Related Products

Human VEGF EasyTest™ ELISA Kit

Mouse VEGF ELISA Kit

Rat VEGF ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Canine VEGF-A ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Canine (dog)

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