Product Name Canine TNF-alpha ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum, Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity <1 pg/ml
Assay Range 2.9-700 pg/ml
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names TNF, TNFA, cTNF
Gene ID 403922
Swissprot ID P51742
|Sample Type||Average % Recovery||Range %|
|Cell culture media||113||97-122|
|Sample Type||Dilution||% of Expected|
|Cell culture media||1:2||103|
Standard Curve: Canine TNF-alpha ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α shares 97% aa sequence identity with rhesus and 71% – 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Canine TNF-alpha ELISA Kit is for Research Use Only.
1 Plate Kit, 2 Plate Kit, 5 Plate Kit