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Rat TWEAK R ELISA Kit
Rat TWEAK R ELISA Kit

Rat TWEAK R ELISA Kit

Catalog No : 3030342


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SKU: 3030342. Category: .

Rat TWEAK R ELISA Kit Features

Product Name            Rat TWEAK R ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   0.25 ng/mL
Assay Range                0.33-80 ng/ml
Reactivity                     Rat
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Tnfrsf12a, Fn14
Gene ID                         302965
Swissprot ID              G3V6F9

Recovery

Sample Type    Average % RecoveryRange %
Serum8779-97
Plasma8580-92
Cell culture media10293-114

Linearity

Sample TypeDilution% of Expected
Serum1:295
1:4112
1:8111
Plasma1:297
1:4101
1:8120
Cell culture media1:2105
1:4104
1:8111

Standard Curve: Rat TWEAK R ELISA Kit

Rat TWEAK R ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

The gene for TNF-related Weak Inducer of Apoptosis Receptor (TWEAK R) was originally identified as the fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts. TWEAK R is the smallest member of the TNF receptor superfamily. This protein is a ligand for the FN14/TWEAKR receptor. Leukocytes are the main source of TWEAK including human resting and activated monocytes, dendritic cells and natural killer cells. TWEAK can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.

Excessive activation of the TWEAK pathway in chronic injury has been described to promote pathological tissue changes including chronic inflammation, fibrosis and angiogenesis. In chronic liver disease for example TWEAK expression is enhanced and causes hepatic stellate cells, which are key regulators of liver fibrosis, to proliferate. NFAT1 regulates the expression of TWEAKR and its ligand TWEAK (this protein) with lipocalin 2 to increase breast cancer cell invasion.

Related Products

Mouse TWAEK R ELISA Kit

Mouse TWEAK ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Rat TWEAK R ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Rat

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