Product Name Rat TWEAK R ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Length 4.5 hours
Sample Volume Serum ,Plasma, cell culture media , 100ul
Detection Method Colorimetric
Sensitivity 0.25 ng/mL
Assay Range 0.33-80 ng/ml
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names Tnfrsf12a, Fn14
Gene ID 302965
Swissprot ID G3V6F9
|Sample Type||Average % Recovery||Range %|
|Cell culture media||102||93-114|
|Sample Type||Dilution||% of Expected|
|Cell culture media||1:2||105|
Standard Curve: Rat TWEAK R ELISA Kit
• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.
The gene for TNF-related Weak Inducer of Apoptosis Receptor (TWEAK R) was originally identified as the fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts. TWEAK R is the smallest member of the TNF receptor superfamily. This protein is a ligand for the FN14/TWEAKR receptor. Leukocytes are the main source of TWEAK including human resting and activated monocytes, dendritic cells and natural killer cells. TWEAK can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.
Excessive activation of the TWEAK pathway in chronic injury has been described to promote pathological tissue changes including chronic inflammation, fibrosis and angiogenesis. In chronic liver disease for example TWEAK expression is enhanced and causes hepatic stellate cells, which are key regulators of liver fibrosis, to proliferate. NFAT1 regulates the expression of TWEAKR and its ligand TWEAK (this protein) with lipocalin 2 to increase breast cancer cell invasion.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Rat TWEAK R ELISA Kit is for Research Use Only.
1 Plate Kit, 2 Plate Kit, 5 Plate Kit