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Rat SCF ELISA Kit

Rat SCF ELISA Kit

Catalog No : 3030023


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SKU: 3030023. Category: .

Rat SCF ELISA Kit Features

Product Name            Rat SCF ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   10 pg/ml
Assay Range               12.3-3000 pg/ml
Reactivity                      Rat
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Kitlg, Kitl, Mgf, SCF
Gene ID                         60427
Swissprot ID               P21581

Recovery

Sample Type    Average % RecoveryRange %
Serum8879-98
Plasma8070-85
cell culture media8977-101

Linearity

Sample TypeDilution% of Expected
Serum1:2116
1:4112
1:8111
Plasma1:2117
1:4101
1:8113
cell culture media1:2121
1:4119
1:8125

Standard Curve: Rat SCF ELISA Kit

Rat SCF ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Human SCF shares 75 – 83% aa sequence identity with canine, feline, mouse, and rat SCF. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF-R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels. CF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF has been shown to increase the survival of HSCs in vitro and contributes to the self-renewal and maintenance of HSCs in-vivo.

Related Products

Human SCF ELISA Kit

Mouse SCF ELISA Kit

Custom canine SCF ELISA kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Rat SCF ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Rat

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