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Rat Leptin ELISA Kit
Rat Leptin ELISA Kit Standard Curve

Rat Leptin ELISA Kit

Catalog No : 3030021


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SKU: 3030021. Category: .

Product Features

Product Name            Rat Leptin ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   10pg/ml
Assay Range               10.9-8000pg/ml
Reactivity                     Rat
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Lep, Ob, obese
Gene ID                        25608
Swissprot ID              P50596

 

Recovery

Sample Type    Average % RecoveryRange %
Serum9283-102
Plasma8476-95
Cell culture media8074-90

Linearity

Sample TypeDilution% of Expected
Serum1:2104
1:4107
1:889
Plasma1:2110
1:4112
1:8101
Cell culture media1:2114
1:498
1:893

Standard Curve: Rat Leptin ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Leptin is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the “hunger hormone”. Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. In obesity, a decreased sensitivity to leptin occurs, resulting in an inability to detect satiety despite high energy stores. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.

Related Products

Human Leptin ELISA Kit

Mouse Leptin ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Rat Leptin ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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