Rat CRP ELISA Kit

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Rat CRP ELISA Kit
Rat CRP ELISA Kit Standard Curve

Rat CRP ELISA Kit

Catalog No : 3030026


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SKU: 3030026. Category: .

Rat CRP ELISA Kit Features

Product Name            Rat CRP ELISA Kit
Assay Type:                  Solid Phase Sandwich ELISA
Format:                          96-well strip plate
Assay Length:             4.5 hours
Sample Volume:        Serum, Plasma, cell culture media , 100ul
Detection Method:   Colorimetric
Sensitivity:                  100 pg/ml
Assay Range:              0.25-60 ng/ml
Reactivity:                    Rat
Cross-Reactivity:      No cross-reactivity observed with available related molecules
Gene Names:              Crp, Aa1249, Ab1-341, Ab2-196, Ac1-114, Ac1262, Ac2-069, Ba2-693
Gene ID                         25419
Swissprot ID               P48199

 

Recovery

Sample Type    Average % RecoveryRange %
Serum9887-108
Plasma117109-132
cell culture media112103-122

Linearity

Sample TypeDilution% of Expected
Serum1:289
1:489
1:897
Plasma1:2102
1:482
1:8102
cell culture media1:2102
1:494
1:898

Standard Curve: Rat CRP ELISA Kit

Rat CRP ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. It induces the phagocytosis of bacteria and apoptotic cells and regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.

Related Products

Human CRP ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Rat CRP ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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