Raf1(Phospho-Ser259) Antibody

Histone H3(Acetyl-Lys23) Rabbit Polyclonal Antibody
PDK1(Phospho-Ser241) Antibody
April 25, 2016
GSK3b Antibody
GSK3a(Phospho-Ser21) Antibody
April 25, 2016
Show all
Histone H3(Acetyl-Lys23) Rabbit Polyclonal Antibody
Raf1(Phospho-Ser259) Antibody
Raf1(Phospho-Ser259) Antibody

Raf1(Phospho-Ser259) Antibody

Catalog No : 11006


Clear
SKU: 11006. Category: .

Product Name

Raf1(Phospho-Ser259) Antibody

Host Species

Rabbit

Clonality

 Polyclonal

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Applications

WB IHC

Reactivity

Hu Ms Rt

Specificity

The antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.

Immunogen

Peptide-KLH. Peptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.

Target Name

Raf1

Alternative Name

C-RAF; C-Raf; CRAF.

Modification

Phospho-Ser259

SDS-PAGE MW

73kd

Accession No

P04049; NP _002871.1

Concentration

1.0mg/ml

Formulation

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.

Application:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.

Sizes

100ul, 50ul

Reviews

There are no reviews yet.

Be the first to review “Raf1(Phospho-Ser259) Antibody”

  • Datasheet