Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Hu Ms Rt
The antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
Peptide-KLH. Peptide sequence around phosphorylation site of serine 259 (S-T-S(p)-T-P) derived from Human Raf1.
C-RAF; C-Raf; CRAF.
P04049; NP _002871.1
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.
Western blotting: 1:500~1:1000
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
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