PDK1(Phospho-Ser241) Antibody

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PDK1(Phospho-Ser241) Antibody
PDK1(Phospho-Ser241) Antibody
PDK1(Phospho-Ser241) Antibody

PDK1(Phospho-Ser241) Antibody

Catalog No : 11005


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SKU: 11005. Category: .

Product Name

PDK1(Phospho-Ser241) Antibody

Host Species

Rabbit

Clonality

 Polyclonal

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Applications

WB IHC IF

Reactivity

Hu Ms Rt

Specificity

The antibody detects endogenous level of PDK1 only when phosphorylated at serine 241.

Immunogen

Peptide-KLH. Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1.

Target Name

PDK1

Alternative Name

PDPK1; PkB kinase; Protein kinase B kinase; hPDK1; kinase PDK1.

Modification

Phospho-Ser241

SDS-PAGE MW

63kd

Accession No

O15530; NP_002604.1

Concentration

1.0mg/ml

Formulation

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.

Application:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Background

Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.

Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63
Chen H, et al. (2001) Biochemistry; 40(39): 11851-9
Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7
Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11

Sizes

100ul, 50ul

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