Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
WB IHC IF
Hu Ms Rt
The antibody detects endogenous level of PDK1 only when phosphorylated at serine 241.
Peptide-KLH. Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1.
PDPK1; PkB kinase; Protein kinase B kinase; hPDK1; kinase PDK1.
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.
Western blotting: 1:500~1:1000
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.
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