NFkB-p65(Ab-468) Antibody

GSK3b Antibody
NFkB-p65(Ab-435) Antibody
April 24, 2016
GSK3b Antibody
NFkB-p65(Ab-536) Antibody
April 24, 2016
Show all
GSK3b Antibody
NFkB-p65 Antibody
NFkB-p65 Antibody

NFkB-p65(Ab-468) Antibody

Catalog No : 21013


Clear
SKU: 21013. Category: .

Product Name

NFkB-p65(Ab-468) Antibody

Host Species

Rabbit

Clonality

 Polyclonal

Purification

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Applications

WB IHC

Reactivity

Hu Ms Rt     

Specificity

The antibody detects endogenous level of total NFkB-p65 protein.

Immunogen

Peptide-KLH. Peptide sequence around aa.466~470 (L-A-S-V-D) derived from Human NFkB-p65.

Target Name

NFkB-p65

Alternative Name

NFKB3; RELA; TF65; Transcription factor p65; p65.

SDS-PAGE MW

65kd

Accession No

Q04206; NP_001138610.1

Concentration

1.0mg/ml

Formulation

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.

Application:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

 

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex

Buss H, et al. (2004) J Biol Chem
Schmitz ML, et al. (2004) Blood

Sizes

100ul, 50ul

Reviews

There are no reviews yet.

Be the first to review “NFkB-p65(Ab-468) Antibody”

  • Datasheet