NFkB-p65(Ab-254) Antibody

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GSK3b Antibody
NFkB-p65 Antibody

NFkB-p65(Ab-254) Antibody

Catalog No : 21010


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SKU: 21010. Category: .

Product Name

NFkB-p65(Ab-254) Antibody
Host SpeciesRabbit
Clonality Polyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIHC
ReactivityHu Ms Rt     
SpecificityThe antibody detects endogenous level of total NFkB-p65 protein.
ImmunogenPeptide-KLH. Peptide sequence around aa.252~256 (F-R-T-P-P) derived from Human NFkB-p65.
Target NameNFkB-p65
Alternative NameNFKB3; RELA; TF65; Transcrip  tion factor p65; p65.
SDS-PAGE MW65kd
Accession NoQ04206; NP_001138610.1
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for a short term use.

Application:

Immunohistochemistry: 1:50~1:100

 

Background

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex

Yeh PY, et al. (2004) J Biol Chem. 279(25): 26143-26148.
Ryo A, et al. (2003) Mol Cell.12 (6): 1413-1426.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.
Haskill S, et al. (1991) Cell 65:1281-1289.

Sizes

100ul, 50ul

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