Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Hu Ms Rt
The antibody detects endogenous level of total NFkB-p100 protein.
Peptide-KLH. Peptide sequence around aa.868~872 (Y-G-S-Q-S) derived from Human NFkB-p100.
DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lyt10; NFKB2
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.
Western blotting: 1:500~1:1000
NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth.
Qu Z, et al. (2004) J Biol Chem; 279(43): 44563-72.
Xiao G, et al. (2001) J Biol Chem 7(2): 401-9.