Mouse VEGF R1 ELISA Kit

Mouse VCAM-1 ELISA Kit
Mouse VCAM-1 ELISA Kit
April 12, 2016
Rat BDNF ELISA Kit
Rat BDNF ELISA Kit
April 12, 2016
Show all
Mouse VEGF R1 ELISA Kit
Mouse VEGF R1 ELISA Kit Standard Curve

Mouse VEGF R1 ELISA Kit

Catalog No : 3020148


Clear
SKU: 3020148. Category: .

Mouse VEGF R1 ELISA Kit Features

Product Name            Mouse VEGF R1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   2 pg/ml
Assay Range               4.1-3000 pg/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Flt1, AI323757, Flt-1, VEGFR-1, VEGFR1, sFlt1
Gene ID                        14254
Swissprot ID              P35969

 

Recovery

Sample Type    Average % RecoveryRange %
Serum10292-113
Plasma9182-101
Cell culture media10598-108

Linearity

Sample TypeDilution% of Expected
Serum1:295
1:480
1:886
Plasma1:297
1:489
1:894
Cell culture media1:2101
1:494
1:892

Standard Curve: Mouse VEGF R1 ELISA Kit

Mouse VEGF R1 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse VEGF R1 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Reviews

There are no reviews yet.

Be the first to review “Mouse VEGF R1 ELISA Kit”