Mouse TIMP-2 ELISA Kit

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Mouse TIMP-2 ELISA Kit
Mouse TIMP-2 ELISA Kit Standard Curve

Mouse TIMP-2 ELISA Kit

Catalog No : 3020013


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SKU: 3020013. Category: .

Mouse TIMP-2 ELISA Kit Features

Product Name           Mouse TIMP-2 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   40pg/ml
Assay Range               0.082-60ng/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Timp2, Timp-2, D11Bwg1104e
Gene ID                        21858
Swissprot ID              P25785

 

Recovery

Sample Type    Average % RecoveryRange %
Serum9280-103
Plasma9382-105
Cell culture media9685-107

Linearity

Sample TypeDilution% of Expected
Serum1:293
1:495
1:887
Plasma1:294
1:492
1:890
Cell culture media1:296
1:489
1:886

Standard Curve: Mouse TIMP-2 ELISA Kit

Mouse TIMP-2 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14. TIMP-2 functions as both an MMP inhibitor and an activator. TIMPs inhibit all active MMPs, but different TIMPs inhibit different MMPs better than other TIMPs. For example, TIMP-1 inhibits MMP-7, MMP-9, MMP-1 and MMP-3 better than TIMP-2, and TIMP-2 inhibits MMP-2 more effectively than other TIMPs. In melanocytic cells TIMP2 gene expression may be regulated by MITF.

Related Products

Human TIMP-2 EasyTest™ ELISA Kit

Rat TIMP-2 ELISA Kit

Canine TIMP-2 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse TIMP-2 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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