Mouse SDF-1 alpha ELISA Kit

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Mouse SDF-1 alpha ELISA Kit
Mouse SDF-1 alpha ELISA Kit Standard Curve

Mouse SDF-1 alpha ELISA Kit

Catalog No : 3020136


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SKU: 3020136. Category: .

Product Features

Product Name            Mouse SDF-1 alpha ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   2pg/ml
Assay Range               9.4-600pg/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Cxcl12, Pbsf, Scyb12, Sdf1, Tlsf, Tpar1
Gene ID                        20315
Swissprot ID              P40224

 

Recovery

Sample Type    Average % RecoveryRange %
Serum9889-106
Plasma9991-110
Cell culture media9790-112

Linearity

Sample TypeDilution% of Expected
Serum1:299
1:498
1:8101
Plasma1:298
1:497
1:896
Cell culture media1:297
1:496
1:895

Standard Curve: Mouse SDF-1 alpha ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. CXCL12 is expressed in many tissues in mice including brain, thymus, heart, lung, liver, kidney, spleen, and bone marrow. CXCL12 is strongly chemotactic for lymphocytes. During embryogenesis, it directs the migration of hematopoietic cells from fetal liver to bone marrow and the formation of large blood vessels. It has also been shown that CXCL12 signalling regulates the expression of CD20 on B cells. CXCL12 is also chemotactic for mesenchymal stem cells and is expressed in the area of inflammatory bone destruction, where it mediates their suppressive effect on osteoclastogenesis.

In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism. CXCR4, previously called LESTR or fusin, is the receptor for CXCL12.This CXCL12-CXCR4 interaction used to be considered exclusive (unlike for other chemokines and their receptors), but recently, it was suggested that CXCL12 may also bind the CXCR7 receptor. By blocking CXCR4, a major coreceptor for HIV-1 entry, CXCL12 acts as an endogenous inhibitor of CXCR4-tropic HIV-1 strains.

Related Products

Human SDF-1 alpha ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse SDF-1 alpha ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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