Mouse Renin 1 ELISA Kit

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Mouse Renin 1 ELISA Kit
Mouse Renin 1 ELISA Kit Standard Curve

Mouse Renin 1 ELISA Kit

Catalog No : 3020134


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SKU: 3020134. Category: .

Mouse Renin 1 ELISA Kit Features

Product Name            Mouse Renin 1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum, Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   5 pg/ml
Assay Range               12.2-50000 pg/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Ren1, D19352, Ren, Ren-1, Ren-Ac, Ren1d, Rn-1, Rnr
Gene ID                        19701
Swissprot ID              P06281

 

Recovery

Sample Type    Average % RecoveryRange %
Serum127112-134
Plasma130123-140
Cell culture media10789-114

Linearity

Sample TypeDilution% of Expected
Serum1:2120
1:4113
1:8111
Plasma1:2116
1:4115
1:898
Cell culture media1:2118
1:4120
1:8101

Standard Curve: Mouse Renin 1 ELISA Kit

Mouse Renin 1 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Renin is a secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat. Mouse Renin is a secreted, 42 – 47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22 – 402).

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Renin 1 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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