Mouse IL-6 ELISA Kit

Mouse VEGF ELISA Kit
Mouse VEGF ELISA Kit
April 10, 2016
Mouse MPO ELISA Kit
Mouse MPO ELISA Kit
April 10, 2016
Show all
Mouse IL-6 ELISA Kit
Mouse IL-6 ELISA Kit Standard Curve

Mouse IL-6 ELISA Kit

Catalog No : 3020018


Clear
SKU: 3020018. Category: .

Mouse IL-6 ELISA Kit Features

Product Name            Mouse IL-6 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   <1pg/ml
Assay Range               0.82-600pg/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Il6
Gene ID                        16193
Swissprot ID              P08505

 

Recovery

Sample Type    Average % RecoveryRange %
Serum9483-104
Plasma9584-106
Cell culture media9279-101

Linearity

Sample TypeDilution% of Expected
Serum1:294
1:493
1:892
Plasma1:296
1:491
1:896
Cell culture media1:293
1:496
1:892

Standard Curve: Mouse IL-6 ELISA Kit

Mouse IL-6 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation.

IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6’s role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.

Related Products

Human IL-6 EasyTest™ ELISA Kit

Rat IL-6 ELISA Kit

Canine IL-6 ELISA Kit

Porcine IL-6 ELISA kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse IL-6 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Reviews

There are no reviews yet.

Be the first to review “Mouse IL-6 ELISA Kit”