Mouse IL-1 beta ELISA Kit

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Mouse IL-1 beta ELISA Kit
Mouse IL-1 beta ELISA Kit Standard Curve

Mouse IL-1 beta ELISA Kit

Catalog No : 3020008


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SKU: 3020008. Category: .

Mouse IL-1 beta ELISA Kit Features

Product Name            Mouse IL-1 beta ELISA Kit
Assay Type:                  Solid Phase Sandwich ELISA
Format:                          96-well strip plate
Assay Length:             4.5 hours
Sample Volume:        Serum ,Plasma, cell culture media , 100ul
Detection Method:   Colorimetric
Sensitivity:                  2 pg/ml
Assay Range:              2.7-2000 pg/ml
Reactivity:                    Mouse
Cross-Reactivity:      No cross-reactivity observed with available related molecules
Gene Names:              Il1b, IL-1beta, Il-1b
Gene ID                         16176
Swissprot ID               P10749

 

Recovery

Sample Type    Average % RecoveryRange %
Serum9989-108
Plasma9889-107
cell culture media9888-110

Linearity

Sample TypeDilution% of Expected
Serum1:298
1:497
1:898
Plasma1:296
1:495
1:897
cell culture media1:289
1:493
1:895

Standard Curve: Mouse IL-1 beta ELISA Kit

Human IL-1 beta ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

IL-1 beta is produced in response to inflammatory agents, infections, or microbial endotoxins. IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia,  atherosclerosis and neuronal injury.

Related Products

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Rat IL-1 beta ELISA Kit

Canine IL-1 beta ELISA Kit

Porcine IL-1 beta ELISA kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse IL-1 beta ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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