Mouse IFN-gamma ELISA Kit

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Mouse IFN-gamma ELISA Kit
Mouse IFN-gamma ELISA Kit Standard Curve

Mouse IFN-gamma ELISA Kit

Catalog No : 3020007


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SKU: 3020007. Category: .

Product Features

Product Name           Mouse IFN-gamma ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   2pg/ml
Assay Range               2.7-2000pg/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               IFNG, IFN-g, Ifg
Gene ID                         15978
Swissprot ID               P01580

Recovery

Sample Type    Average % RecoveryRange %
Serum9584-103
Plasma9482-112
cell culture media9389-101

Linearity

Sample TypeDilution% of Expected
Serum1:290
1:494
1:890
Plasma1:292
1:495
1:892
cell culture media1:293
1:491
1:892

Standard Curve: Mouse IFN-gamma ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

IFN-gamma is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. FNγ is secreted by T helper cells (specifically, Th1 cells), cytotoxic T cells (TC cells) and NK cells only. IFNγ is the only Type II interferon and it is serologically distinct from Type I interferons; It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects.

In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Related Products

Human IFN-gamma EasyTest™ ELISA Kit

Canine IFN-gamma ELISA Kit

Porcine IFN-gamma ELISA kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse IFN-gamma ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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