Mouse Galectin-3 ELISA Kit

Mouse Flt-3L ELISA Kit
Mouse Flt-3L ELISA Kit
April 11, 2016
Mouse IL-10 ELISA Kit
Mouse IL-10 ELISA Kit
April 11, 2016
Show all
Mouse Galectin-3 ELISA Kit
Mouse Galectin-3 ELISA Kit Standard Curve

Mouse Galectin-3 ELISA Kit

Catalog No : 3020069


Clear
SKU: 3020069. Category: .

Mouse Galectin-3 ELISA Kit Features

Product Name            Mouse Galectin-3 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   4 pg/ml
Assay Range               8.2-2000 pg/ml
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Lgals3,GBP, L-34, Mac-2, gal3
Gene ID                        16854
Swissprot ID              P16110

 

Recovery

Sample Type    Average % RecoveryRange %
Serum115102-125
Plasma9892-109
Cell culture media120115-130

Linearity

Sample TypeDilution% of Expected
Serum1:290
1:480
1:890
Plasma1:2101
1:482
1:892
Cell culture media1:2102
1:485
1:889

Standard Curve: Mouse Galectin-3 ELISA Kit

Mouse Galectin-3 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Galectin-3 is a protein that in humans is encoded by the LGALS3 gene. Galectin-3 is a member of the lectin family, of which 14 mammalian galectins have been identified. The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. Galectin-3 (Gal-3) is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis.

Related Products

Rat Galectin-3 ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse Galectin-3 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Reviews

There are no reviews yet.

Be the first to review “Mouse Galectin-3 ELISA Kit”