Mouse Galectin-3 EasyTest™ ELISA Kit

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Mouse Galectin-3 EasyTest™ ELISA Kit
Mouse Galectin-3 EasyTest ELISA Standard Curve

Mouse Galectin-3 EasyTest™ ELISA Kit

Catalog No : 1020069


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SKU: 1020069. Category: .

Product Features

Product Name           Mouse Galectin-3 EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   20 pg/mL
Assay Range                46.8-3000pg/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Lgals3, GBP, L-34, Mac-2, gal3
Gene ID                         16854
Swissprot ID               P16110

 

Recovery

Sample Type    Average % RecoveryRange %
Serum10592-121
Plasma9786-108

Linearity

Sample TypeDilution% of Expected
Serum1:294
1:492
1:891
Plasma1:295
1:489
1:894

Standard Curve: Mouse Galectin-3 EasyTest™ ELISA Kit

Mouse Galectin-3 EasyTest ELISA Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim mouse Galectin-3 mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

Galectin-3 is a protein that in humans is encoded by the LGALS3 gene. It is a member of the lectin family, of which 14 mammalian galectins have been identified. Human and mouse Galectin-3 shares approximately 80% amino acid sequence similarity. Galectin-3 has an affinity for beta-galactosides and exhibits antimicrobial activity against bacteria and fungi. It has been shown to be involved in the following biological processes: cell adhesion, cell activation and chemoattraction, cell growth and differentiation, cell cycle, and apoptosis.

Galectin-3 associates with the primary cilium and modulates renal cyst growth in congenital polycystic kidney disease. A correlation between galectin-3 expression levels and various types of fibrosis has been found. Galectin-3 is upregulated in cases of liver fibrosis, renal fibrosis, and idiopathic pulmonary fibrosis. Elevated levels of galectin-3 have been found to be significantly associated with higher risk of death in both acute decompensated heart failure and chronic heart failure populations.

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Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Mouse Galectin-3 EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

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