Mouse CT-1 ELISA Kit

Mouse CRG-2 ELISA Kit
Mouse CRG-2 ELISA Kit
September 7, 2016
Mouse CTACK ELISA Kit
Mouse CTACK ELISA Kit
September 7, 2016
Show all
Mouse CT-1 ELISA Kit
Mouse CT-1 ELISA Kit Standard Curve

Mouse CT-1 ELISA Kit

Catalog No : 3020050


Clear
SKU: 3020050. Category: .

Mouse CT-1 ELISA Kit Features

Product Name            Mouse CT-1 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   150 pg/mL
Assay Range                0.25-60 ng/mL
Reactivity                     Mouse
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               Ctf1, CT-1
Gene ID                         13019
Swissprot ID               Q99JA0

 

Recovery

Sample Type    Average % RecoveryRange %
Serum  8175-92
Plasma 8377-94
Cell culture media  9485-107

Linearity

Sample TypeDilution% of Expected
Serum1:2101
1:496
1:892
Plasma1:295
1:497
1:8102
Cell culture media1:289
1:493
1:888

Standard Curve: Mouse CT-1 ELISA Kit

Mouse CT-1 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Mouse CT-1 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Mouse

Reviews

There are no reviews yet.

Be the first to review “Mouse CT-1 ELISA Kit”