MEK2(Phospho-Thr394) Antibody

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MEK2(Phospho-Thr394) Antibody
MEK2(Phospho-Thr394) Antibody

MEK2(Phospho-Thr394) Antibody

Catalog No : 11008


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SKU: 11008. Category: .

Product Name

MEK2(Phospho-Thr394) Antibody

Host Species

Rabbit

Clonality

 Polyclonal

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Applications

WB IHC IF

Reactivity

Hu

Specificity

The antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.

Immunogen

Peptide-KLH. Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2.

Target Name

MEK2

Alternative Name

ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK/ERK kinase 2; MAPKK 2

Modification

Phospho-Thr394

SDS-PAGE MW

44kd

Accession No

P36507; NP_109587.1

Concentration

1.0mg/ml

Formulation

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for a short term use.

Application:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.

Crews C M, et al. (1992) Science. 258:478-480.
Alessi D R, et al. (1994) EMBO J. 13:1610-1619.
Rosen L B, et al. (1994) Neuron. 12:1207-1221.
Cowley S, et al. (1994) Cell. 77:841-852.

Sizes

100ul, 50ul

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