Human VEGF R3 EasyTest™ ELISA Kit

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Human VEGF R3 EasyTest™ ELISA Kit
Human VEGF R3 ELISA Kit Standard Curve

Human VEGF R3 EasyTest™ ELISA Kit

Catalog No : 1010017


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SKU: 1010017. Category: .

Product Features

Product Name           Human VEGF R3 EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   15pg/mL
Assay Range                65.5-16000pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               FLT4; FLT-41; LMPH1A; PCL; VEGFR-3; VEGFR3
Gene ID                         2324
Swissprot ID               P35916

 

Recovery

Sample Type    Average % RecoveryRange %
Serum   9478-106
Plasma    109107-111

Linearity

Sample TypeDilution% of Expected
Serum1:291
1:473
1:886
Plasma1:291
1:475
1:882

Standard Curve: Human VEGF R3 EasyTest™ ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human VEGF R3 mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human VEGF R3 EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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