Human VEGF EasyTest™ ELISA Kit

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Human VEGF EasyTest™ ELISA Kit
Human VEGF Easytest ELISA Kit Standard curve

Human VEGF EasyTest™ ELISA Kit

Catalog No : 1010015


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SKU: 1010015. Category: .

Product Features

Product Name           Human VEGF EasyTest™ ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Process             One wash step, two incubations
Assay Length              1.5 hours
Sample Volume         Serum , Plasma, 50µL
Detection Method    Colorimetric
Sensitivity                   20 pg/pg/mL
Assay Range               40.9-10000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               VEGFA; MVCD1; VEGF; VPF
Gene ID                         7422
Swissprot ID               P15692

Recovery

Sample Type    Average % RecoveryRange %
Serum    11291-128
Plasma    9791-108

Linearity

Sample TypeDilution% of Expected
Serum1:2130
1:4141
1:8122
Plasma1:2122
1:4130
1:8123

Standard Curve: Human VEGF EasyTest™ ELISA Kit

Human VEGF Easytest ELISA Kit Standard curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Bioaim human VEGF mix
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 µl of standard or sample to each well.
3. Add 50 µl of  Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 µl of Stop Solution to each well.
8. Read at 450 nm/570 nm.

Background

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis. In addition to binding to VEGFRs, VEGF binds to receptor complexes consisting of both neuropilins and VEGFRs. This receptor complex has increased VEGF signalling activity in endothelial cells. Neuropilins are pleitrophic receptors and therefore other molecules may interfere with the signalling of the NRP/VEGFR receptor complexes.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status      

Human VEGF EasyTest™ ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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