Human VEGF-C ELISA Kit

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Human VEGF-C ELISA Kit
Human VEGF-C ELISA Kit Standard Curve

Human VEGF-C ELISA Kit

Catalog No : 3010149


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SKU: 3010149. Category: .

Product Features

Product Name            Human VEGF-C ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method    Colorimetric
Sensitivity                   10pg/ml
Assay Range               13.7-10000pg/ml
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               VEGFC, MVCD1, VEGF, VPF
Gene ID                        7422
Swissprot ID               P49767

 

Recovery

Sample Type    Average % RecoveryRange %
Serum10999-118
Plasma9686-106
Cell culture media9282-102

Linearity

Sample TypeDilution% of Expected
Serum1:2112
1:4104
1:8111
Plasma1:299
1:496
1:893
Cell culture media1:2105
1:4102
1:897

Standard Curve: Human VEGF-C ELISA Kit

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a VEGF subfamily that shares the conserved VEGF homology domain (VHD) with other VEGF family members, but is distinguished by their preferential formation of non-covalent homodimers. The main function of VEGF-C is in lymphangiogenesis, where it acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth and migration. It was shown to be a specific growth factor for lymphatic vessels in a variety of models. However, in addition to its effect on lymphatic vessels, it can also promote the growth of blood vessels and regulate their permeability. The effect on blood vessels can be mediated via its primary receptor VEGFR-3 or its secondary receptor VEGFR-2.

Related Products

Human VEGF EasyTest™ ELISA Kit

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human VEGF-C ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

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