Human Tie-2 ELISA Kit

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Human Tie-2 ELISA Kit
Human Tie-2 ELISA Kit Standard Curve

Human Tie-2 ELISA Kit

Catalog No : 3010292


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SKU: 3010292. Category: .

Human Tie-2 ELISA Kit Features

Product Name            Human Tie-2 ELISA Kit
Assay Type                   Solid Phase Sandwich ELISA
Format                           96-well strip plate
Assay Length              4.5 hours
Sample Volume         Serum ,Plasma, cell culture media , 100ul
Detection Method   Colorimetric
Sensitivity                   15 pg/mL
Assay Range                20.6-15000 pg/mL
Reactivity                     Human
Cross-Reactivity       No cross-reactivity observed with available related molecules
Gene Names               TEK, CD202B, TIE-2, TIE2, VMCM, VMCM1
Gene ID                         7010
Swissprot ID               Q02763

 

Recovery

Sample Type    Average % RecoveryRange %
Serum11092-122
Plasma10293-108
Cell culture media8070-89

Linearity

Sample TypeDilution% of Expected
Serum1:284
1:492
1:890
Plasma1:291
1:486
1:883
Cell culture media1:290
1:487
1:889

Standard Curve: Human Tie-2 ELISA Kit

Human Tie-2 ELISA Kit Standard Curve

Kit Components

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Standards
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Protocol Outline

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Wash and add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Wash and add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Wash and add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm/570 nm.

Background

Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). Ang-2 has been reported to act as an antagonist for Ang-1.This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. Tie-2 is involved in vascular stabilization and remodeling.  Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Regulatory Status 

Human Tie-2 ELISA Kit is for Research Use Only.

Sizes

1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Species Detected

Human

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